| 质粒名称: | MIP 247 CoMiP 4in1 with shRNA p53 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 63726 |
| 存储实验室: | Mark Kay, Joseph Wu |
|
载体骨架基本信息 |
|
| 载体名称: | Mark Kay, Joseph Wu |
| 载体出品公司: | Joseph Wu's Lab at Stanford University |
| 空载体大小: | 8062 bp |
| 完整质粒大小: | 9411bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | RNA-OUT |
| 载体抗性: | RNA-OUT ( 6% sucrose) |
| 生长温度: | 30 ℃ |
| 宿主菌: | RNA-OUT selection competent cells, such as NTC4862 DH5α competent cells (Nature Technology) |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Mini-intronic-plasmid intron、pUC-RNA-OUT、MIP intron |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1355 bp |
| 基因突变: | codon-optimised |
| 启动子: | SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53 |
| 标签/融合蛋白: | IRES-dtomato (C terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI (not destroyed) |
| 3’克隆位点: | AgeI (not destroyed) |
| 5’测序引物 | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | MIP 247 CoMiP 4in1 with shRNA p53 was a gift from Mark Kay & Joseph Wu (Addgene plasmid # 63726 ; http://n2t.net/addgene:63726 ; RRID:Addgene_63726) |
| 参考文献部分: | Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC. Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081. 10.1038/srep08081 PubMed 25628230 |
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MIP 247 CoMiP 4in1 with shRNA p53载体质粒基本信息
MIP 247 CoMiP 4in1 with shRNA p53质粒图谱载体图谱和MIP 247 CoMiP 4in1 with shRNA p53载体序列质粒序列多克隆位点信息
MIP 247 CoMiP 4in1 with shRNA p53质粒载体简介
MIP 247 CoMiP 4in1 with shRNA p53质粒序列载体序列
LOCUS MIP 247 CoMiP 4in1 with shRNA p53 9411 bp DNA circular SYN 14-APR-2025
DEFINITION To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single
plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9411)
AUTHORS Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW,
Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
TITLE Novel codon-optimized mini-intronic plasmid for efficient,
inexpensive, and xeno-free induction of pluripotency.
JOURNAL Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
PUBMED 25628230
REFERENCE 2 (bases 1 to 9411)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep.";
date: "2015-01-28"; volume: "5:8081. doi"; pages: "
10.1038/srep08081"
COMMENT Sequence Label: MIP 247 CoMiP 4in1 with shRNA p53
FEATURES Location/Qualifiers
source 1..9411
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 46..452
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
primer_bind complement(578..595)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(749..1337)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(829..848)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
regulatory 1827..1836
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2922..2978
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4401..4454
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 5415..5474
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS 5478..6794
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQSELQPPAPS
EDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTEL
LGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNP
ARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSL
LSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSP
SAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRK
CTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKAT
AYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
misc_feature join(6829..7403,7404..7406,7407..7415)
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(6996..7013)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 7223..7242
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7416..8117
/codon_start=1
/product="dimeric variant of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=dTomato
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLYGMDELYK"
primer_bind complement(7549..7567)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 7817..7836
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 8011..8034
/label=DsRed1-C
/note="DsRed1, forward primer"
misc_feature 8136..8724
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8189..8209)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8607..8618)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 8736..8960
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(9118..9138)
/label=mU6-F
/note="Mouse U6 promoter, forward primer"
ORIGIN
1 ggctccccgg gcgcgactag tgaattcaga tctgagctag ctgcagtaac gccattttgc
61 aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga
121 aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg
181 ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc
241 cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc
301 ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct
361 tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc
421 gcgccagtcc tccgacagac tgagtcggcc ggtggatcca ccggtattgg gatcttcaca
481 cagcaggtaa ggttgcgggc cgggcctggg ccgggtccgg gccgggtatt gcccgcctaa
541 tgagcgggct tttttttctt accccttctt ccgcttcctc gctcactgac tcgctgcgct
601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
661 cagaatcagg ggataacgca ggaaagaacg tgtgagcaaa aggccagcaa aaggccagga
721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
1021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
1081 acttatcgcc actggcagcg gccactggta acaggattag cagagcgagg tatgtaggcg
1141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
1201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttggtccg
1261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
1321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
1381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
1441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
1501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
1561 catccatagt tgcctgactc ctgcaaacca cgttgtggta gaattggtaa agagagtcgt
1621 gtaaaatatc gagttcgcac atcttgttgt ctgattattg atttttggcg aaaccatttg
1681 atcatatgac aagatgtgta tctaccttaa cttaatgatt ttgataaaaa tcattaggta
1741 ccccggcccg cactgacccc tggtgttgct tttttttttt aggccgcaag ctgaagcgtg
1801 tccactagct gactagctaa accggtgcca ccatggccgg acacctggcc agcgacttcg
1861 cctttagccc acctcctggc ggagggggag atggacctgg cggccctgaa cctggctggg
1921 tggaccctcg gacctggctg tcctttcaag gccctccagg cggacctgga attggaccag
1981 gcgtgggccc tggcagcgaa gtgtggggca tccctccctg ccctcccccc tacgagtttt
2041 gcggcggcat ggcctactgt ggacctcagg tcggcgtggg actggtgcct cagggcggcc
2101 tggaaacctc tcagcctgag ggcgaagccg gcgtgggcgt ggagagcaac tctgacggcg
2161 ccagccctga gccttgtacc gtgaccccag gcgccgtgaa gctggaaaaa gagaagctgg
2221 aacagaaccc cgaggaaagc caggacatca aggccctgca gaaagaactg gaacagttcg
2281 ccaagctgct gaagcagaag cggatcaccc tgggctacac acaggctgac gtgggactga
2341 cactgggcgt gctgttcggc aaggtgttca gccagaccac catctgcaga ttcgaagccc
2401 tgcagctgtc cttcaagaac atgtgcaagc tgcggcccct gctgcagaaa tgggtggagg
2461 aagccgacaa caacgagaac ctgcaggaaa tctgcaaggc cgagacactg gtgcaggccc
2521 ggaagcggaa gcggaccagc atcgagaaca gagtgcgggg caacctggaa aacctgttcc
2581 tgcagtgccc caagcccacc ctgcagcaga tcagccacat tgcacagcag ctgggactgg
2641 aaaaggacgt cgtgagagtg tggttctgca accggcggca gaagggcaag agaagcagca
2701 gcgactacgc ccagagagag gacttcgagg ccgctggctc cccttttagc ggcggacccg
2761 tgtcctttcc tctggcccct ggccctcact ttggcacccc tggctacggc agcccacact
2821 tcaccgccct gtacagcagc gtgccctttc ccgagggcga ggccttccct cctgtgtccg
2881 tgaccaccct gggcagcccc atgcatagca actcttccgg agccaccaac ttctccctgc
2941 tgaagcaggc cggcgacgtg gaggagaacc ccggcccctc catggctgtg tccgacgccc
3001 tgctgcccag cttcagcacc tttgcctccg gccctgctgg cagagagaaa acactgcggc
3061 aggctggcgc ccctaacaac agatggcggg aggaactgag ccacatgaag cggctgcctc
3121 ccgtgctgcc cggcagacct tatgatctgg ccgctgccac cgtggccacc gatctggaat
3181 ctggcggagc cggcgctgcc tgtggcggaa gcaacctggc ccccctgccc agacgggaga
3241 cagaggaatt caacgacctg ctggacctgg acttcatcct gagcaacagc ctgacccacc
3301 ctcctgagtc tgtggccgcc accgtgtcta gcagcgccag cgccagcagc agctctagcc
3361 ctagcagcag cggacctgcc agcgccccca gcacctgcag cttcacctac cccatcagag
3421 ccggcaatga ccctggcgtg gcccctggcg gaacaggagg aggactgctg tacggcagag
3481 agtccgcccc tccccccacc gcccccttca acctggccga catcaacgat gtgtccccca
3541 gcggcggctt tgtggccgag ctgctgcgac ctgagctgga ccccgtgtac atcccccctc
3601 agcagccaca gcctcccggc ggaggactga tgggcaagtt cgtgctgaag gccagcctga
3661 gcgcccctgg cagcgagtac ggcagcccca gcgtgatcag cgtgtccaag ggcagccctg
3721 atggctctca ccctgtggtg gtggcccctt acaatggcgg ccctcccaga acctgcccca
3781 agatcaagca ggaagccgtc agcagctgta cacacctggg agccggccct ccactgagca
3841 atggccacag acctgccgcc cacgacttcc ctctgggaag gcagctccct agcagaacca
3901 cccctaccct gggcctggaa gaggtgctgt ccagcagaga ctgccaccct gccctgcctc
3961 tgccacctgg ctttcaccct caccctggcc ccaactaccc cagcttcctg cccgaccaga
4021 tgcagcctca ggtgcccccc ctgcactacc aagaactgat gcccccaggc agctgcatgc
4081 ccgaggaacc caagcccaag cggggcagaa gaagctggcc ccggaagaga accgccacac
4141 acacctgcga ttacgccggc tgcggcaaga cctacaccaa gagcagccac ctgaaggccc
4201 acctgagaac ccacaccggc gagaagccct accactgcga ctgggacggc tgtggctgga
4261 agttcgccag aagcgacgag ctgacccggc actacagaaa gcacaccggc caccggccct
4321 tccagtgcca gaagtgcgac cgggccttca gcagaagcga ccacctggct ctgcacatga
4381 agagacactt cggctccgga gagggccggg gctctctgct gacctgtggc gacgtggagg
4441 agaaccccgg cccctccatg tacaacatga tggaaaccga gctgaagccc cctggccctc
4501 agcaaacatc tggcggcgga gggggcaatt ctacagccgc cgctgccggc ggaaaccaga
4561 agaacagccc cgacagagtg aagcggccca tgaacgcctt catggtctgg tcccgcggcc
4621 agcggcggaa gatggcccaa gaaaacccca agatgcacaa cagcgagatc agcaagagac
4681 tgggcgccga gtggaagctg ctgtccgaga cagagaagag acccttcatc gacgaggcca
4741 agcggctgag agccctgcac atgaaagagc accccgacta caagtaccgg cccagaagaa
4801 agaccaagac cctgatgaag aaggacaagt acaccctgcc tggcggactg ctggcccctg
4861 gcggcaattc tatggccagc ggagtgggag tgggagctgg actgggagcc ggcgtgaacc
4921 agcggatgga cagctacgcc cacatgaacg gctggtccaa cggcagctac agcatgatgc
4981 aggaccagct gggctaccct cagcaccctg gcctgaatgc ccatggcgcc gctcagatgc
5041 agcccatgca cagatacgac gtgtccgccc tgcagtacaa cagcatgacc agcagccaga
5101 cctacatgaa tggcagcccc acctacagca tgtcctacag ccagcagggc acacctggca
5161 tggccctggg cagcatgggc agcgtggtca agagcgaggc cagcagcagc cctcctgtgg
5221 tcaccagcag ctcccacagc agagcccctt gtcaggccgg cgacctgcgg gacatgatca
5281 gcatgtacct gccaggcgcc gaagtgcctg aacctgccgc ccctagcaga ctgcacatga
5341 gccagcacta ccagagcggc cctgtgcctg gcaccgccat caatggcacc ctgcccctga
5401 gccacatgac gcgtcagtgc acaaactacg ccctgttgaa gctggctgga gacgtggagt
5461 ccaaccctgg accctccatg cccctcaacg ttagcttcac caacaggaac tatgacctcg
5521 actacgactc ggtgcagccg tatttctact gcgacgagga ggagaacttc taccagcagc
5581 agcagagcga gctgcagccc ccggcgccca gcgaggatat ctggaagaaa ttcgagctgc
5641 tgcccacccc gcccctgtcc cctagccgcc gctccgggct ctgctcgccc tcctacgttg
5701 cggtcacacc cttctccctt cggggagaca acgacggcgg tggcgggagc ttctccacgg
5761 ccgaccagct ggagatggtg accgagctgc tgggaggaga catggtgaac cagagtttca
5821 tctgcgaccc ggacgacgag accttcatca aaaacatcat catccaggac tgtatgtgga
5881 gcggcttctc ggccgccgcc aagctcgtct cagagaagct ggcctcctac caggctgcgc
5941 gcaaagacag cggcagcccg aaccccgccc gcggccacag cgtctgctcc acctccagct
6001 tgtacctgca ggatctgagc gccgccgcct cagagtgcat cgacccctcg gtggtcttcc
6061 cctaccctct caacgacagc agctcgccca agtcctgcgc ctcgcaagac tccagcgcct
6121 tctctccgtc ctcggattct ctgctctcct cgacggagtc ctccccgcag ggcagccccg
6181 agcccctggt gctccatgag gagacaccgc ccaccaccag cagcgactct gaggaggaac
6241 aagaagatga ggaagaaatc gatgttgttt ctgtggaaaa gaggcaggct cctggcaaaa
6301 ggtcagagtc tggatcacct tctgctggag gccacagcaa acctcctcac agcccactgg
6361 tcctcaagag gtgccacgtc tccacacatc agcacaacta cgcagcgcct ccctccactc
6421 ggaaggacta tcctgctgcc aagagggtca agttggacag tgtcagagtc ctgagacaga
6481 tcagcaacaa ccgaaaatgc accagcccca ggtcctcgga caccgaggag aatgtcaaga
6541 ggcgaacaca caacgtcttg gagcgccaga ggaggaacga gctaaaacgg agcttttttg
6601 ccctgcgtga ccagatcccg gagttggaaa acaatgaaaa ggcccccaag gtagttatcc
6661 ttaaaaaagc cacagcatac atcctgtccg tccaagcaga ggagcaaaag ctcatttctg
6721 aagaggactt gttgcggaaa cgacgagaac agttgaaaca caaacttgaa cagctacgga
6781 actcttgtgc gtaagtcgac gaattcggta ccgcgggccc gggatccgcc cctctccctc
6841 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
6901 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
6961 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
7021 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
7081 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
7141 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
7201 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
7261 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
7321 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
7381 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggaggtca
7441 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
7501 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
7561 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
7621 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
7681 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
7741 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
7801 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
7861 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
7921 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
7981 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
8041 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca
8101 tggacgagct gtacaagtaa agcggccgcg tcgacaatca acctctggat tacaaaattt
8161 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
8221 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
8281 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
8341 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
8401 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
8461 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
8521 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
8581 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
8641 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
8701 tctccctttg ggccgcctcc ccgcctggac tcgagctgtg ccttctagtt gccagccatc
8761 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
8821 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
8881 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
8941 ggatgcggtg ggctctatgg agatctgata tctctagtcc cgggctgcag gaattccaaa
9001 aaagactcca gtggtaatct actctcttga agtagattac cactggagtc ggcccaaaca
9061 aggcttttct ccaagggata tttatagtct caaaacacac aattacttta cagttagggt
9121 gagtttcctt ttgtgctgtt ttttaaaata ataatttagt atttgtatct cttatagaaa
9181 tccaagccta tcatgtaaaa tgtagctagt attaaaaaga acagattatc tgtcttttat
9241 cgcacattaa gcctctatag ttactaggaa atattatatg caaattaacc ggggcagggg
9301 agtagccgag cttctcccac aagtctgtgc gagggggccg gcgcgggcct agagatggcg
9361 gcgtcactag agtcgaccca tgggggcccg ccccaactgg ggtaaccttt g
//
